As part of the BBSRC-funded project Designing Future Wheat, NIAB has generated several hexaploid wheat nested association mapping (NAM) populations using two broad sources of wheat genetic diversity:
(a) tetraploid wheat sources of genetic variation captured in a hexaploid wheat genetic background via directly crossing tetraploid accessions (AB subgenomes) with elite hexaploid wheat cultivars (ABD subgenomes). The resulting hexaploid germplasm are termed Tetraploid/Hexaploid (TetHex) lines and were used to make the Spring (S) and Winter (W) TET NAMs.
(b) creation of synthetic hexaploid wheat (SHW), which capture novel genetic variation from the diploid wheat relative Aegilops tauschii (D genome) combined with T. durum wheat (AB genome) to create SHW (ABD subgenomes), which are then crossed with elite cultivars (ABD subgenomes) to make the Spring (S) and Winter (W) SHW NAMs.
Using these sources of variation, NIAB has generated the following NAM populations:
- NIAB_SW_TET_NAME: consisting of 58 bi-parental populations, created by crossing 58 tetraploid accessions (Triticum durum, T. dicoccum, T. dicoccoides) to the bread wheat T. aestivum spring cultivar Paragon, to generate 1,784 recombinant inbred lines (RILs).
- NIAB_WW_TET_NAM: consisting of 21 bi-parental populations, created by crossing 21 tetraploid accessions to the bread wheat winter cultivar Robigus, to generate 646 RILs.
- NIAB_SW_SHW_NAM: consisting of 64 bi-parental populations, created by crossing 64 SHW accessions to the bread wheat spring cultivar Paragon, to generate 4,200 RILs.
- NIAB_WW_SHW_NAM: consisting of 55 bi-parental populations, created by crossing 54 SHW accessions to the bread wheat winter cultivar Robigus, to generate 3,241 RILs.
Seed availability
As part of DFW, the above germplasm will be deposited at the SeedStor at John Innes Centre, UK. For seed inquiries in the interim contact Dr Phil Howell.
Genotypic data
35k single nucleotide polymorphism (SNP) array genotypic data is available for the NIAB_SW_TET_NAM and NIAB_WW_SHW_NAM at the NIAB GitHub.